28 A. In whole transcriptome sequencing experiments we should not exprect to see

Question

28 A. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment

True

False

28 B. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs

True

False

Explanation / Answer

28.A False. we can observe the expression of all genes on the genome.

28.B. mRNA sequencing can identify both known and novel transcript isoforms, gene fusions and allele-specific expression. It gives complete information on the coding transcriptome and is not restricted by the presence or absence of prior knowedge.

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