38. The region of the Drosophila genome containing the bw gene is diagrammed bel
ID: 181093 • Letter: 3
Question
38. The region of the Drosophila genome containing the bw gene is diagrammed below. The position and direction of the arrow labeled "bw" ndicate the transcription start site (TSS) and transcription termination site for the bw RNA. (Point- 3' end ofRNA) 23540k 23520k 23530k Gene Span RabX1 mi ppk3 Gr 59f Gr59e Wrmd a. The DNA sequence corresponding to the TSS region (beginning) of the bw gene is shown below. Which strand (top or bottom) will be the template for transcription (circle one)? Cl pt.] 5' CG AGCCGCCGGAT ATT TCGA GCTA 3 TOP TCTTGCA 3' GCT CGGCGGCOTAAGAACGTAAAGCTCGAT 5' BOTTOM b. Write the sequence of the mRNA that is transcribed from the template strand of the bw gene. Label the 5' end of the mRNA that you draw. [3 pts.] 5' u AG cucGAA AuG CAA GAA, ucc GGCGGcuca 3 can be written 3 y 5 c. Using the table of the Genetic Code, translate the coding region of the mRNA that you drew. Label the amino end (N-terminus) of the polypeptide sequence. [4 pts. 2 N Met other Ser AA cerrect a correct order Second letter for term UUU Phenyl alanine UAU UGU Cysteine UCU UAC Tyrosine UCC Serine UCA Stop codon UGA Stop codon A UUA UAA term UUG Leucine UCG Stop codon UGG Tryptophan G UAG CAC Histidine CGU CGC CCU CCA Proline CGA Arginine CAG Glutamine CGG AUU ACU AUC Isoleucine ACC AGU Asparagine AGC Serine AAC A AUA Threonine AGA AUG e ACG Lysine AGG Arginine AAG start codon Aspartic GAC acid GUA Valine Alanine GAA Glutamic GGG LIFE 9e, Figure 14.6 Continue to next page. 10Explanation / Answer
Answer:
(a)
The bottom strand (3’--->5’) is the template
template strand refers to the sequence of DNA that is copied during the synthesis of mRNA.
The top strand (5’-->3’) is the coding strand (mRNA like strand)
(B)
5’CGAGCCGCCGGAUUCUUGCAUUUCGAGCUA 3’
(c)5’CGA GCC GCC GGA UUC UUG CAU UUC GAG CUA 3’
N terminus-Arg-Ala-Ala-Gly-Phe-Leu-His-Phe-Glu-Leu-C terminus
(d)
Wild type mRNA 5’CGA GCC GCC GGA UUC UUG CAU UUC GAG CUA 3’
Mutant mRNA 5’CGA GCC GCC GGA UUA UUG CAU UUC GAG CUA 3’
Mutated protein:
N terminus-Arg-Ala-Ala-Gly-Leu-Leu-His-Phe-Glu-Leu-C terminus
The above mutation causes A missense mutation: It refers to a base substitution when the change in nucleotide changes the amino acid coded for by the affected codon.
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