Regarding Sanger\'s sequencing method, why does the incorporation of a ddNTP in

Question

Regarding Sanger's sequencing method, why does the incorporation of a ddNTP in the growing complementary strand terminates the process of DNA replication?

a. Because the ddNTP cannot form hydrogen bonds with the complementary base in the template strand due to the missing hydroxyl group (–OH).

b. Because the ddNTP cannot form a peptide bond with the complementary base in the template strand due to the missing hydroxyl group (–OH).

c. Because the ddNTP cannot form a phosphodiester bond with the sugar from a newly recruited dNTP due to the missing hydroxyl group (–OH).

Explanation / Answer

c. Because the ddNTP cannot form a phosphodiester bond with the sugar from a newly recruited dNTP due to the missing hydroxyl group (–OH).

The ddNTPs lack 3' hydroxyl group which is required for the formation of phosphodiester bond with the sugar group of the incoming nucleotide. This leads to termination of extension by DNA polymerase .

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