3, (5 points) From the figure below, what can you conclude about how the protein

Question

3, (5 points) From the figure below, what can you conclude about how the proteins SVP, FLM-B and FLM-5 interact with the DNA sequence of the SEP3 gene? It is important to note the relative protein sizes: monomers of SVP are 40 kDa and monomers of both versions of FLM proteins are 15 kDa in size. The weight of the SEP3 probe is negligible. In your answer be sure to identify which proteins are bound to the SEP3 probe DNA for each band that appears on the figure. 175 150 100 Figure 8 EMSA using SEP3 probe (32P-labeled DNA obtained by amplifying the SEP3 gene). The probe was incubated with proteins as indicated. Numbers indicate approximate sizes in kDa. SEP3 probe

Explanation / Answer

An EMSA test is used to investigate the relationship between between a genetic sequence or DNA and the protein in order to investigate the extent of binding of the protein with DNA. This type of experiment is ususally conducted to understand the effect of a regulatory protein on gene expression.

According to the diagrammatic information, it can be clearly seen that the protein FLM beta binds very strongly with the probe thus giving 4 bands in the membrane. On the other hand, FLM delta binds slightly weakly with the probe thus giving only 2 bands. However, since the two bands of FLM delta are similar to those of FLM beta, it is very likely that both these subunits bind to each other during gene regulation and then modulate gene expression.

Thus, this information is suggestive of a fact that FLM beta and delta are co-regulatory proteins for the probe of DNA whereby the DNA sequence is the target for dimer of FLM delta and teteramer of FLM beta.

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