QUESTION 4 Given that you set up your PCR reactions correctly, would you expect
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QUESTION 4 Given that you set up your PCR reactions correctly, would you expect to see a band for GMO positive control (plant) template DNA amplified with PSll primers and with GM primers? Choose the single, best answer. OA PSll yes, GM yes OB. PSII no, GM no OC.PSIl no, GM yes OD PSll yes, GM no QUESTION 5 What are advantages to using PCR? Choose all that apply. A Amplifies specific DNA sequences exponentially B. Sensitive to contaminating DNA in sample C.PCR is fast OD We need to know the DNA sequeefet to generate specific primers E Works with trace amount of template DNA QUESTION 6 Given that you set up your PCR reactions correctly, would you expect to see a band for non-GMO test food (plant) template DNA amplified with PSIl primers and with GM primers? Choose the single, best answer:Explanation / Answer
4) Option A : PS II and GM yes is the correct answer.
GMO positive control plant template DNA will give a band when amplified with PS II primer and GM primer. The presence of band with PS II primer is used to verify that viable plant DNA was extracted as PS II is a primer to universal plant gene, Photosystem II. GM primer is used to very that the modified gene is present in the DNA sample. So, in a properly set PCR reaction, both PS II primer and GM primer will give a band in GMO positive control DNA.
5) Options A, C, D and E are the correct options.
The basic function of PCR is amplification of a specific DNA template. In PCR, the DNA is amplified in the exponential manner of 2n with 'n' being the number of PCR cycles carried out. PCR is fast and is capable of working with trace amount of DNA with the end result being the amplification of the template DNA. Also, knowledge of the template sequence is essential for generating correct primers which ensure the specificity and sensitivity of DNA.
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