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Determine the map distances in recombination units Bacterial Mapping Problem A c

ID: 18877 • Letter: D

Question

Determine the map distances in recombination units

Bacterial Mapping Problem A cross is made between two E. coli strains: Hfr: a+b+c+ (a, b, and c are nutritional requirements) F-: a- b- c- Interrupted-mating studies show that a+ enters the recipient last, so a+ recombinants are selected on a medium containing b and c only. The selected cells are a+ recombinants (can grow without a). The a+ recombinants are tested for the presence of b+ and c+. The following numbers of colonies are found for each genotype.

Explanation / Answer

answer : gene order a b c

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