Consider a dissociation reaction between a protein and its ligand: PL P + L. Whi

Question

Consider a dissociation reaction between a protein and its ligand: PL P + L. Which of following would help you measure the dissociation constant (Kd) for the reaction? xd Determine the concentration of L required for all molecules of P to be bound to L B Measure the amount of PL formed at equilibrium as a function of increasing L-equ C Measure the rate of dissociation as a function of increasing amounts of L. D Measure the concentration of P and L at equilibrium E Mix equal amounts of P and L and then measure the rate of association.

Explanation / Answer

for the determination of P and l dissociation we have to measure the p and L concentration at equilibrium.

in the equilibrium condition the concentration of P and l determines the rate of dissociation at which the dissociates and releases from PL complex.

option d is correct

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