Ps 3 tor Canwas (1Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MA

Question

Ps 3 tor Canwas (1Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILINGS REVIEWVIEW Cut AaBbCcD AaßbCeD AaßbCeD AaßbCeD AaBbCcD AaBbCcl Paste. Cnpy B , u, kex, x A,. . :EE , ,n· 11Normal 1N0 Spac Replace Select . Heading 1 Heading 2 Title Subtitle Subtle Em Emp hasis Intense E Strong Ouote Format Painter sly es Problem Set 3 Genetics/Agronomy 320 Spring 2018Name, uestion 1·(8 pts). Consider that the following double stranded DNA is your gene of interest. Please indicate the mRNA sequence that will be generated ater transcription of your gene and the polypeptide generated atter translation. Use the bottom strand as your template strand. Label the 5, and 3, of the mRNA and the amino ("N") and carboxylC") ends of the polypeptide. Use the genetic code ta to generate your polypeptide (page 108 of texl) 5 CGCTATGAATCCAATCAAGCATGAATAGTTT 3 3 GCGATACTTAGGTTAGTTCGTACTTATCAAA 5 DNA a. (1 pt) mRNA: b. 1 pt) polypeptide: C. (1 pt) What is the sequence of bases of the anticodon indicate 5' and 3 ends of the anlicodon) for the third amino acid of that polypeptide? d. (1 pt) How many codois are used to produce that palpeptide? Mark them in your mRNA strand e (1 pt) How many anticodons are used to produce that polypeptide? f. (1 pt) Mark the 5UTR sequence In the GIVEN DNA g. (1 pt) Mark the 3 UTR sequence in the GIVEN DNA h. (1 pt) How many BNAs will be used to make this palypeptide? PAGE 1 OF 3 ous vrcRU5 1114 AM Type here to search

Explanation / Answer

DNA TEMPLATE STRAND 3' GCGATACTTAGGTTGATTCGTACTTATCAAA 5'

mRNA 5' CGCU AUG AAUCCA ACU AAG CAU GAA UAGUUU 3'

POLYPEPTIDE Start codon AUG and stop codon UAG, UUU

N- Met Asn Pro Thr Lys His Glu - C

Third amino acid is Proline so its anticodon will be 5' UGG 3'

7 codons are used to make this polypeptide

3nucleotides = 1codon (codons are underlined inthe mRNA strand)

7 anticodons and 7 tRNAs are used

The bold nucleotide sequences are the 5' UTR and 3' UTR region, respectively.

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