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You are hired to design the front end of an imaging system for studying the boun

ID: 1922767 • Letter: Y

Question

You are hired to design the front end of an imaging system for studying the boundary
shapes of cells, bacteria, viruses, and protein.The front end consists, in this case,
of the illumination source(s) and corresponding imaging camera(s).The diameters
of circles required to enclose individual specimens in each of these categories
are 50, 1, 0.1, and 0.01 m, respectively.
(a) Can you solve the imaging aspects of this problem with a single sensor and
camera? If your answer is yes, specify the illumination wavelength band and
the type of camera needed. Identify the camera as being a color camera, farinfrared
camera, or whatever appropriate name corresponds to the illumination
source.
(b) If your answer in (a) is no, what type of illumination sources and corresponding
imaging sensors would you recommend? Specify the light sources and cameras as requested in part (a). Use the minimum number of illumination
sources and cameras needed to solve the problem.
By solving the problem we mean being able to detect circular details of diameter : 50,1,0.1 and 0.01 micrometer respectively.

Explanation / Answer

The source of the illumination required to see an object must have wavelength the same size or smaller than the object. Because interest lies only on the boundary shape and not on other spectral characteristics of the specimens, a single illumination source in the far ultraviolet (wavelength of .001 microns or less) will be able to detect all objects. A farultraviolet camera sensor would be needed to image the specimens. (b) No answer required since the answer to (a) is affirmative.

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