Calculate the volume of pBS-SK+ plasmid needed for tubes 1C (C = control) and 2R

Question

Calculate the volume of pBS-SK+ plasmid needed for tubes 1C (C = control) and 2R (R = restriction) to
get a final concentration of 100 µg/ml. You will be given a pBS-SK+ plasmid DNA with a concentration
of 200 µg/ml. After calculating the volume, change ml to µl and round off the calculation to the nearest
microliter.
C1 = initial concentration of plasmid (200 µg/ml)
V1 = ? (volume of plasmid needed)
C2 = final concentration (100 µg/ml)
V2 = final volumes (either 0.025 ml or 0.200 ml)

tube 1C: C1 x V1 = C2 x V2
_____ x V1 = 100 µg/ml x 0.025 ml
V1 = ______ml = ______ µl

tube 2R: C1 x V1 = C2 x V2
_____ x V1 = 100 µg/ml x 0.200 ml
V1 = ______ml = ______ µl

Explanation / Answer

we know that V1 * C1 = C2 * V2

Gven that for tube 1C,

C1 = 200microgram/ml

C2 = 100 microgram/ ml

V2 = 0.025ml for tube 1C, 0.200ml for tube 2R

therefore, V1 = 100 * 0.025 / 200 = 0.0125ml = 12.5 microlitre

for tube 2R, V1 = 100 * 0.200 / 200 = 1ml = 1000microlitre.

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