After cloning the Citrate Synthase gene you consulted the genome databases and f

Question

After cloning the Citrate Synthase gene you consulted the genome databases and find that the human Citrate Synthase enzyme (found in the Krebs Cycle) primary conformation begins with the amino acid sequence Met-Trp-Lys-Trp- Trp-Met. But the protein made from your plasmid begins with Met-Trp-Met-Trp-Trp-Met! A mutation must have occurred! Write out the most likely DNA sequence of the non-templated strand for the beginning of the actual (unmutated) Citrate Synthase gene. Don’t forget 5 and 3 ends.

Explanation / Answer

Unmutated Amino Acid Sequence : Met-Trp-Lys-Trp-Trp-Met

Mutated Amino Acid Sequence : Met-Trp-Met-Trp-Trp-Met

Mutated mRNA :5' AUG-UGG-AUG-UGG-UGG-AUG 3'

Now the codons that code for Lys are AAG and AAA

Therefore, possible sequence for unmutated mRNA

5' AUG-UGG-AAG-UGG-UGG-AUG 3'

5' AUG-UGG-AAA-UGG-UGG-AUG 3'

Thus in the unmutated mRNA following sequence is there

5' AUG-UGG-AAG-UGG-UGG-AUG 3'

unmutated template strand sequence : 3' TAC-ACC-TTC-ACC-ACC-TAC 5'

unmutated non - template strand sequence :5' ATG-TGG-AAG-TGG-TGG-ATG 3'

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