As you were setting up your PCR reaction to amplify a specific fragment of DNA,

Question

As you were setting up your PCR reaction to amplify a specific fragment of DNA, you became distracted by your lab mates (Dr. Bunsen and Beaker), who accidently lit Beeker’s hair on fire. You added all the ingredients for PCR (Template DNA, Taq, dNTPs, a buffer) except you only added one primer instead of both. You then set up the reaction to cycle 30 times.

a. Will this reaction produce a billion copies of double stranded DNA? Explain. (2 pts)

b. Assuming that you started with only on copy of template DNA in your PCR reaction, how many copies of the target loci on each strand will you make after the reaction is finished? (3 pts)

Explanation / Answer

Answer:- PCR is a technique which is known to be polymerase chain reaction and this technique is to used to amplify the Billion of copies of DNA... As PCR is very a sensitive technique which pay a attention at the time of preparation... As if content are missed or concentration is either more or less, it will not amplified the accuracy of DNA fragments.. So if primers are not added, means only one primer is added no billion of copies of copies are formed, as only single stranded copies of DNA are formed. As primers are added from both the stranded from 5' to 3' and 3 ' to 5'.

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