Lab exercise 12 Identification of an unknown enteric bacterium Introduction: Ent
ID: 195434 • Letter: L
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Lab exercise 12 Identification of an unknown enteric bacterium Introduction: Enteric microbes are those that occur in the intestines. Escherichia coli is an enteric of which most of us have heard. It has served as a model organism to understand bacterial metabolism & genetics and has served as a representative of the bacterial world. In general, it is non-pathogenic Gram-negative rod that colonizes the intestines of humans and other vertebrates. It can ferment a wide variety of carbohydrates and metabolize many different organic molecules. Taxonomically, it belongs to the family Enterobacteriaceae. It is easy to isolate and identify in the laboratory. Many of you will isolate E coli in this exercise; however it is possible to isolate other interesting enteric species Goal: 1. To identify an enteric bacterium Procedure Materials: One TSB slant per student with different enteric bacteria One RapID™ ONE System kit per student .Here is the link for a video on how to use the kit: https://vimeo.com/109132372 Protocol Follow the RapID™ ONE System One you have recorded your code, follow instructions at: http/llremel.com/ERIC 1, 2. ERIC is the database User name: ewumicro Password: isitecoli a. b. Identifications c. Choose ONE d. Submit Report your results After analyzing your results, you will hopefully be able to identify the enteric bacteria as:Explanation / Answer
1. Germ-free animals are the animals having no microorganisms including bacteria, viruses, fungi, protozoa, and parasites, living in or on them that means they are free of ALL microorganisms, including those that are typically found in the gut throughout its lifetime. whereas When one or more known species of microorganisms are added experimentally to a germfree plant or animal, the host, is known as the animal with microbes. In some animals, microbes are found in symbiotic relationship. So the major difference between germ free and animal with microbes is the presence or absence of microbes in or on the animal body.
2. The more diverse the diet, the more diverse the microbiome and the more adaptable it will be to perturbations. Like all healthy ecosystems, richness of microbiota species characterizes the GI microbiome in healthy individuals. Conversely, a loss in species diversity is a common finding in several disease states. This biome is flooded with energy in the form of undigested and partially digested foods, and in some cases drugs and dietary supplements. Each microbiotic species in the biome transforms that energy into new molecules. Therefore, the diverse microbiome awould be a healthier microbiome.
3. An enterotype is a classification of living organisms based on its bacteriological ecosystem in the gut microbiome. The gastro-intestinal tract, especially the gut, is rich in different microorganisms, which play important role in our health and diseases. There are four main enterotypes: Parabacteroides, Prevotella, Ruminococcus, and Bacterioides. Understanding our gut microbiome may help us to increase the precision of disease prediction, their classification and treatment.
4. Dysbiosis is also known as dysbacteriosis which means microbial imbalance or maladaptation on or inside the body because friendly bacteria are critically important for the health of our digestive and immune systems, for their detoxification and hormone-regulating capabilities, and for nutrient formation and absorption. Dysbiosis of the gut microbiota is associated with the pathogenesis of both intestinal and extra-intestinal disorders. Intestinal disorders include inflammatory bowel disease, irritable bowel syndrome, and coeliac disease, while extra-intestinal disorders include allergy, asthma, metabolic syndrome, cardiovascular disease, and obesity.
There are four general causes of intestinal dysbiosis: putrefaction, fermentation, deficiency and sensitization.
The most common symptoms that suggest your gut bacteria is out of whack include:
Cure: Take probiotics every day.
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