3. Triphenyltetrazolium chloride is not universally added to motility medium bec

Question

3. Triphenyltetrazolium chloride is not universally added to motility medium because it actually inhibits the motility of some organisms. If a bacterium tests positive for motility when using a motlity tube containinyg confident in your results? What if it tests negative? REFERENCES Cowan, M.K. 2012. Microbiology: A Systems Approach, 3rd ed., chap. 3. New York: McGraw-Hill. Talaro, K.P, and Chess, B. 2015. Foundations in Microbiology, 9th ed, chap. 3. New York: McGraw-Hill. Wilty lu Sherwood. Iu and Woolverton G 2014. Prescott, Harley&Klein;'s Microbiology, 9th ed., chap. 21. New York: McGraw-Hil

Explanation / Answer

Answer 3. Triphenyltetrazolium chloride is actually 2,3,5-Triphenyl-tetrazolium chloride solution which is commonly used in the autopsy pathology for identifying myocardial infarctions. In layman language, we can also say that it assists the post mortem studies. This chemical is also used for testing biochemical viability in the seeds. Living cells turn red with it and non living cells remain colorless. This is quite a potent chemical.

No, we cannot be confident in our results if bacteria shows motility with triphenyltetrazolium chloride, beacuse as already stated this chemical is quite reactive and stops 100% motility.

If the test is negative then it may lead to wrong conclusions, especially if it is related to autopsy.

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