A model of an IgG antibody is given in the figure below. Assuming that each Heav

Question

A model of an IgG antibody is given in the figure below. Assuming that each Heavy chain(H) is about 50 kD and each Light chain(L) is about 25 kD, predict the number of bands and their apparent molecular masses that would appear using each of the following techniques:

Gel Filtration:

SDS-PAGE:

Reducing SDS-PAGE:

Antigen-binding site Antigen-binding N site NH VH Heavy chain (H) regions (V GH1 Hinge region S-S CH1 Light chain (L) CL Sr S-S C CH2 CH2 Constant regions (C) Interchain CH3 disulfide bonds CH3 This figure is from the following website: http://apbrwww5.apsu.edu/thompson/Anatomy%20&% 20Physiology/2020/2020%20Exam%20 Reviews/Exam%2 02/CH 21%20anti body. structure-giant-diagram. htm

Explanation / Answer

The answer to the above mentioned problem is mentioned below

Gel filtration - A single band would appear at 150 kD.

SDS page- A single band would appear at 150 kD.

Reducing SDS page- two bands would appear each at 50 kD and 25 kD.

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