IV. Digital image analysis (DIA) is a common technique to quantify the DNA conte

Question

IV. Digital image analysis (DIA) is a common technique to quantify the DNA content in the cell nuclei on tissue and cells, which may help distinguish benign and malignant cells. As cell proliferation is one of the hallmarks in cancer cells, a higher DNA content can serve as an important indicator for cancer diagnosis. In DIA, cach nucleus of the cell on the slide are stained with an absorbing dye called Feulgen stain, which only binds to DNA, followed by the quantitative image analysis to measure the amount of DNA inside the cell nuclei. (1) Please propose the best type of microscope to use for this application. (2 points)

Explanation / Answer

Fluorescent imaging methods, used with either confocal or wide-field microscopy, are used for 2D or 3D images collection of of the cell nucleus.

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