Results of Southern blot analyses of DNA extracted from two B cell lymphomas, an

Question

Results of Southern blot analyses of DNA extracted from two B cell lymphomas, and a T cell lymphoma cell line and digested by a restriction enzyme are given below. Assume that the lowest bands on each blot represent the germline condition C mu probe A B C C kappa probe 1) Which lane corresponds to Icell DNA results?--what condition of antibody or immunoglobulin gene segments is demonstrated by these cells: 2) Which lane (s) could correspond to a B cell stage with IgM containing a lambda chain: 3) Which lane (s) sould correspond to a B cell stage with IgM containing a kappa chain: 4) Which lane(s) appear to be demonstrating allelic exclusion? Circle the lane letter(s) below: kappa: A BC mu: A B C 5) Which lanes indicate somatic recombination of DNA that is definitely coding for an antibody chain? A BC 6) Which of A, B or C could correspond to a pre-B cell stage? A BCNone 7) Which lane has a pattern of bands that theoretically could correspond to a plasma cell stage secreting IgE: Are the SC exon sequences expressed (used to code for amino acids)? Yes or No What type of light chain(s) would it have?

Explanation / Answer

Answer 1. Lane B corresponds to T cell DNA results.  As T-cells genes are similar to B-cell genes and contain multiple V, D and J gene segments in their beta chains and also V and J gene segments in their alpha chains. During the development lymphocyte genes of T-cell rearranged and provide that cell an antigen receptor.

Answer 2. Lane c with c muprobe

Answer 3. Lane c with c kappa probe

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