Restriction enzymes are used to construct restriction maps of DNA. These are dia

Question

Restriction enzymes are used to construct restriction maps of DNA. These are diagrams of specific DNA molecule that show the sites where the restriction enzymes cleave the DNA. To construct a restriction map, purified samples of the DNA are treated with restriction enzymes, either alone or in combination, and then the reaction products are separated by agarose gel electrophoresis, a technique that separates the DNA fragments based on size. (The smaller fragments travel more quickly and are found near the bottom of the gel, whereas the larger fragments are found near the top of the gel.)

Use the results of the agarose gel electrophoresis separation (above) to construct a restriction map for the sample of DNA.

Explanation / Answer

Restiction enzymes should be so designed for the cloning purposes that it should take the whole sequence from the insert and the same enzymes must also be present in the MCS without any sidturbance in the plasmid structure.

If the restriction cuts only once in the insert, than one linearized plasmid will be observed. If it cuts twice, or the cut is made with the restriction enzymes used for cloning, than the insert and the plasmid, two band will be visible, upper one is plasmid and down will be the insert. Here it cannot be predicted because the picture of gel is either not uploaded or not in visible format in the browser.

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