Restriction mapping of a linear piece of DNA reveals the following EcoR1 restric

Question

Restriction mapping of a linear piece of DNA reveals the following EcoR1 restriction sites, along with the sizes of the resulting DNA fragments:

       EcoR1 site 1           EcoR1 site 2

                          ____________________________________________________________________________

       2 kb           4 kb               5 kb

a. This piece of DNA is cut by EcoR1, the resulting fragments are separated by gel electrophoresis, and the gel is stained to visualize those DNA fragments. Draw a picture of the banding pattern that will appear in the gel, and indicate the size of the bands.

b. If a mutation that alters the EcoR1 site 1 occurs in this piece of DNA, draw the expected banding pattern, along with the size of the bands.

NORM MUTANT

------- ---------- ORIGIN

Explanation / Answer

Electrophoresis separates molecules on the basis of charge and mass.larger fragment will move less and slower.As in the first case 3 fragments will be produced one of 2 kb,4kb and 5 kb.so the 2kb fragment will travel more.

if the mutation takes place than two fragments will be produced 6kb (4kb+2kb) and 5 kb.

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