In an attempt to increase yields of the protein erythropoietin (EPO), you have c

Question

In an attempt to increase yields of the protein erythropoietin (EPO), you have changed the ribosome
binding site to more closely base-pair with the large 16S ribosomal sub-unit. Write simple material
balances to determine the changes in [mRNA] and [protein].
a. What is the new [protein]ss how does it compare to the old?
b. How long does it take to reach the new steady-state (or, say, 99% of the way)?

Cell volume 10^-15 L
Cell doubling time 20 minutes
Transcription initiation rate, h 10 sec-1
mRNA degradation rate, d 0.2 min-1
Protein half-life 90 min
Old translation initiation rate 0.04 sec-1
New translation initiation rate 0.5 sec-1

Explanation / Answer

Erythropoitin or EPO is glycoprotein cytokine. Its main function is to stimulate the production of Red blood cell in bone marrow. It is producedin kidney but during fetal period it is produced in liver.

It is produced in the interstitial fibroblast in kidney where kidney secrete erythropoietin which increase the production of red blood cells. In absence of anemia the levl of EPO in blood is low. Low RBC in blood lead to reduction in oxygen level in tissues and when this reduction is detected by kidney then EPO is produced.

During this EPO binds to and activate the receptor on erythroid progenitor cells.

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