Chrome File Edit View History Bookmarks People Window Help 4) us. 100%Sun Mar 11

Question

Chrome File Edit View History Bookmarks People Window Help 4) us. 100%Sun Mar 11 11:39 PM Q E Take BIO314 2rn Secure https://portal.utoronto.ca/webapps/assessment/take/launch.jsp?course-assessment_id=-161651-1&course-ide-92; 7293_1&new-attempt; a : Apps Maria Robinson qu…Watch Arsenal vs s.. r Sports TV 1-Wate.. Management l Ran. m Fred Perry Footwea V sports-Live Video CE is.byu.edu/courses. o -Other Bookmarks Question Complietion Status My Grades Contacts Library Reeources QUESTION 1 1 points You conducted a cloning experiment where you wanted to ligate a piece of DNA into a vector and then transform E. coli with the product of this reaction. You saw colonies on your positive control for the transformation plate. You did not observe any colonies cn your experimental plate, positive control for ligation plate, nepative control for ligation plate or negative control for transformation plate. What is the most likely reason for these cbservations and the failure of the experiment? Your sample was 1st uring purification. O The ligase enzyme was not trional. The sample DNA was not camp etaly digested. The cells were not kept on ice prior to transformation. Your insen and pasmid do not have compatible ends. QUESTION 2 2 points Save Answor Which of the follawing components found in a typical PCR master mix is/are consumed ie. became part of the amplfied product) during the reaction? Select all the options that apply. Buffer DNA polymerase Primers dNTPs water QUESTION 3 1 points Save Answer After performing PCR, you run samples af your positive control, negative contral, and experimental reactions on an agarase gel but fail to see bands in any of these lanes, but your DNA ladder is visible. Which of the folowing options could NOT possibly explain your lack of PCR product DNA polymerase was not adced The annealing temperature was too high The extension time was too brief. The dNTP mix did not include ATFP The gel dd nat include any stain. You used the bufer option wth no Mge Cick Sove and Submit to s0ve ard sthmit. Csck Sqve AM Answers to suve al anawers Sawe Al Anewera Save and Submit

Explanation / Answer

1. Option B is correct.
Since colonies were not obtained in the ligation positive plate, it appears that ligase enzyme is not functional.

2. Option E is correct.
dNTPs become incorporated into the PCR products.

3. Option E is correct.
Since ladder bands could be observed on the gel, it is clear that the gel contains the stain.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.