Chrome File Edit View History Bookmarks People Window Help 4)-Lu.s. 100%Sun Mar

Question

Chrome File Edit View History Bookmarks People Window Help 4)-Lu.s. 100%Sun Mar 11 11:40 PM Q E Take BIO314 2rn Secure https://portal.utoronto.ca/webapps/assessment/take/launch.jsp?course-assessment_id=-161651-1&course;_id=927293_1&new-attempt-1; : Apps Maria Robinson qu…Watch Arsenal vs s.. r Sports TV 1-Wate.. Management l Ran, m Fred Perry Footwea V sports-Live Video CE is.byu.edu/courses. o -Other Bookmarks Question Completion Status: Oxford Nanopore Single Molecule Real Time (SMRT) 1, 3& 4 None of the above QUESTION 7 1 points Save Answer In the T7 expression system, IPTG is used to induce expression of bacterially encoded T7 RNA polymerase, which in turn transcribes a plasmid encoded target gene located downstream of the T7 promoter. True False QUESTION 8 5 points Save Answer Which of the following are possible problems you might encounter when trying to express a eukaryotic protein within a prokaryotic system? Check all the options that apply The prokaryote organism may use different codons preferentially for certain amino acids than the eukaryote from which the protein The prokaryote cannot process transcripts containing introns The eukaryote protein might not fold corectly inside the prokaryote cell The protein requires post-translational modifications to become functional and these do not exist in the prokaryote. The eukaryote protein may have unintended 'toxic' effects in the prokaryote cell. Click Save and Submit to save and submit. Click Save All Answers to saue all ansavers. Save and Submit

Explanation / Answer

Questions 7:

Answer is false because iptg is used as alternate to lactose, which is used by lactose operon. Hence lactose or iptg can be used for those systems only which have lack promoter. So to have its own promoter so it cannot be used in to.

Question 8:

1. May have different colons for few amino acid

2.prokaryotes can not handle intros as the are found only in eukaryotes

3. There is no proper folding mechanism in prokaryotes so eukaryotic protein will not fold properly

4.there is no proper mechanism for post translationAl modifications in prokaryotes

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.