In an assay, supercoiled DNA was incubated with a DNA replication related enzyme

Question

In an assay, supercoiled DNA was incubated with a DNA replication related enzyme over a 5 minute time period. Aliquots were removed at 0, 0.25, 0.5,1,1.5, 2, 3 and 5 minutes. Each aliquot was electrophoresed in a 1% agarose gel and the DNA visualized by staining with ethidium bromide. The results are shown in Figure 7.4. 0 0.25 0.5 1.0 1.5 2.0 3.0 5.0 Time (min) Of the following, the most relaxed DNA is found at what time period? 48 Figure 7.4 A. 0 minutes B. 0.25 minutes C. minute D. 1.5 minutes E. 5 minutes

Explanation / Answer

Supercoiled DNA are more compact and smaller in size than relaxed DNA. So, relaxed DNA experience more frictional resistance in gel and hence migrate slower than supercoiled DNA. The most relaxed DNA is found in the lane with 1 minute of aliquot.

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