Suppose that you are studying an inducible operon that contains two genes requir

Question

Suppose that you are studying an inducible operon that contains two genes required for the metabolism of compound xyz. Gene A encodes enzyme A, and gene B encodes enzyme B. When compound xyz is absent, the operon is repressed, but when compound xyz is present, the operon is induced You have isolated four distinct mutants, numbered 1, 2, 3, and 4, which have the effects shown in the table below, where + indicates that the enzyme is synthesized and- indicates that the enzyme is not synthesized. Identify the component of the operon that is mutated in each mutant. xyz absent Enzyme A Enzyme B Enzyme A Enzyme B Component xyz present Wild type Mutant 1 Mutant 2 Mutant 3 Mutant 4 gene A repressor gene B promoter Incorrect Whereas the components mutated in other mutants may also be incorrect, you have switched the components mutated in mutants 1 and 3. The genes in an ope Therefore, if one of the enzymes is synthesized but the other is not, the gene that encodes the missing ron are transcri d as a single mRNA enzyme must be mutated.

Explanation / Answer

The Mutant 2 is mutated in each component that is the Gene B. Mutating even XYZ is Absent.

XYZ Absent XYZ Present Enzyme A Enzyme B Enzyme A Enzyme B Wild Type - - + + Mutant 1 - - + - Mutant 2 + + + + Mutant 3 - - - + Mutant 4 - - - -

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