Exercise# 16 Title: Expectation (purpose): List Equipment/Tools: Results (Attach

Question

Exercise# 16 Title: Expectation (purpose): List Equipment/Tools: Results (Attached) NOTE: attached observations must include recordkeeping requirements, observations, labelled diagrams Questions from Exercise 16 1. What method was used to perform the Acid-Fast Stain? 2. What makes Mycobacterlum resistant to staining? 3. What is the primary stain in the acid-fast stain? 4. What is the secondary stain in the acid-fast stain? 5. What is the color of Mycobacterium after the addition of the secondary stain? 6. Which staining procedure would be most useful in staining the Leprosy bacillus; Gram Stain, Spore Stain, Simple Stain, Negative Stain, Capsular Stain, Flagella Stain, or Acid- Fast Stain? 7. What is the advantage of the Kinyoun method over the Ziehl-Neelsen method? 8. Mycolic Acid is a cell wall ipid, which of the following cells has mycolic acid; a. Mycobacterium tuberculosis b. Mycobacterium leprae c. All of the above contain mycolic acid d. None of the above contain mycolic acid 1 l Page 1Pa ge

Explanation / Answer

1) the method was not given in the experiment so i preferred in kinyoun method

2) mycobacterium has a well sorrounded waxy cell wall which contains mycolic acid. hear mycolic acid is a complex lipid that prevents call from penetrating

3)the primary stain used hear was basic fuchsin

4) secondary stain was methylene blue

5) after the addition of secondary stain the color will be pink

6)the stain useful for leprosy bacillus was acid fast staining

7) the advantages of kinyoun method was it does not having heating in the procedure so there will be no releasing of noxious phenol fumes

8)the answer was both the species consisting of mycolic acid which is responsible for resistance

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