8. (4 points) Match the role of each cloning vector feature with its function. C

Question

8. (4 points) Match the role of each cloning vector feature with its function. Choose the BEST letter for each term. Some letters may not be used at all. If no answer is available, leave the space blank. stop codon a. enables selection for plasmid-containing cells b. enables screening for insert-containing plasmids c. its protein product attracts RNA polymerase to the promoter d. DNA sequence recognized by RNA polymerase and transcription laczZ gene promoter ori multiple cloning site -lactamase gene pBAD factors e. signal for RNA polymerase to release the template strand f. guides the DNA polymerase to its start site g. contains multiple unique restriction sites transcriptional terminator h. no tRNA binding site i. inducible promoter

Explanation / Answer

Stop codon: No tRNA binding site

lacZ gene: Enables selection for insert+vector containing cells (Insertional inactivation).

Promoter: It is a DNA sequence element recognized by RNA polymerase and other transcription factors.

Ori: It is the site of replication origin. It guides DNA polymerase to initiate replication.

MCS: Contains multiple restriction sites used for cloning

Beta-lactamase gene: Enables selection for the plasmid containing cells.

pBAD: It is an inducible promoter

Transcriptional terminator: It is the site where RNA polymerase releases the template strand.

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