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NCBI BLAST = http://blast.ncbi.nlm.nih.gov/Blast.cgi 1. Using the unknown sequen

ID: 215465 • Letter: N

Question

NCBI BLAST = http://blast.ncbi.nlm.nih.gov/Blast.cgi

1. Using the unknown sequence provided at the bottom, perform a BLASTN (i.e., nucelotide blast) search using “Database” set to the "RefSeq Genome Database (refseq_genomes)" and “Organism” set to “vertebrates (taxid:7742):

Also, make sure the Program Optimization setting is set to "Highly similar sequences (megablast)":

Keep all other settings and parameters at the default value.

1.a. How many Blast Hits do you get for the sequence?  

Answer =

1.b. What is the sequence most similar to? Provide the top-listed sequence Accession as your answer.

Answer =

1.c. What is the E-value for this sequence and is it significant and why?

Answer =

1.d. Are there any other results having the same E-value as this sequence? If so, how many?

Answer =

1.e. For the other results having the same E-value as this sequence, how many also have the same Query coverage?

Answer =

1.f. For those results having the same E-value and same Query coverage, do they also have the same Total score?

Answer =

>Unknown_Sequence

GATATTCACATGATCGCAAATGACCCGCGGCTCCGGCGGCAGCGTCTCAAGCGCCCTCCGCCCGCTCGCAGCAGCAGTAAGTGTGCACGACTGTGCAAGTGTGAGAGCATCATGGTAGCATTCAAAGGAGTCTGGACTCATCCCTTCTGGAAAGCCGTTTCAGCAGAATTTTTGGTCATGTTGATTTTTGTCCTCCTCAGCCTTGGCTCTACGATCAACTGGGGTGGATCAGAGAAGCCACTGCCCGTAGACATGGTCCTTATCTCTCTCTGCTTTGGACTGAGCATTGCAACCATGGTGCAGTGCTTTGGACACATCAGCGGTGGCCACATCAACCCTGCTGTGACTGTGGCCATGGTCTGCACAAGAAAGATCAGCCTCGCCAAATCGGTCTTCTACATTCTTGCCCAGTGCCTGGGAGCCATCGTGGGAGCTGGCATCCTCTACCTCATCACACCACCGAGTGTGGTGGGAGGCCTGGGAGTCACTGCGGTACACGGGGATCTTTCCGCTGGCCATGGACTCCTGGTGGAGCTGATAATTACATTTCAGCTGGTTTTTACTATTTTTGCCAGCTGTGATTCAAAACGAAGTGATGTCACTGGTTCAGTAGCTCTAGCAATTGGATTTTCTGTTGCAATTGGACATTTATTTGCTATCAATTACACTGGTGCCAGTATGAACCCTGCTCGCTCATTTGGACCTGCTGTCATCATGGGAAAATGGGAAAACCAATGGGTTTATTGGGTGGGGCCAATAATAGGAGCAGTCCTTGCTGGTGCTCTTTATGAGTATGTCTATTGCCCAGACGTGGAGCTCAAACGCCGTTTTAAAGATGTCTTCAGTAAAGCTACTCAGCCATCCAAAGGGAAGTACATAGAAGTGGATGACACCAGGAGCCACGTAGAGACCGATGACCTGATCCTGAAGCCTGGCATTGTCCACGTGATTGATATTGACAGGAGTGAGGACAAGAAGGGAAGAGATCCATCAAGTGAGGTGCTGTCTTCTGTATGACTAGCAAGGAGCACTGAAAGCAGAGAGCAGCCTGCCAGCGACTCCACAGATATCCTTCCACCTATCAAACAGAGAGCAGCCTGCCAGCGACTCCACAGATATCCTTCCACCTATCAAAGAAACAGATCTCCTCTAAACAGAGCATCTATCATTTCTTAAAAAGTGTGGTGAAGGCAGCTGTGTGGTAGTGGCATCACCAAACCATACATCTGCTCAGCTGGAATATTAGGACTTCATTATAATTAGGATTCCCCACGAATTATTCTAAATTTGGAGGTGTTCCTGCAATTTTCCTTCCTTTCTTTCTGGAACAACCCCAAAGTCAAAAAGAGATGAAAGCACTCTTCTTTAATAAATCAGTCAATAATGAGATGAAGATAGAGCTGTTTAACATTCAGATTGACAGATAAGATGTATCAGGAAATGCCTATAGACATGAAGACCTACTTATCAGATTGTTCTTCTGACACTTAATTGACTGTTGTCATCTTTGATTAGAACATCTTATCCATTAAGCATTCTCTGTGAGGTTCAGGGACAGCACCAACAGTATTTAACAGTTTTATCCAAAGTCAAGCAATGGAGTATTGTTTCCACTTCACGTATGTCACTACTACCTTGCAAACTACCTCTGAAATAAATGATATTTTAATAGGCTCCAGAAAAAAATTCGATCAACCCATCAAATTTCACTCATACGATTTTCTGTATAAATGTATTACCTTCATCTCTTCCCAGGAGTAAATATGCTGAAATTGAATATTGAAGTCTACCGTAATAAGGCTGCAAAGCATTTAACTGTACTTCATGCTCACTTTTGACATTGTCTATCTGGTGAAACATTCTCCTGGGGTTTGACTATTGACCATTTCATGTTAGCAGACTCTCAAGGTCACG

Choose Search Set C Human genomic+transcript CMouse genomic+ transcript Others (nr etc.) Refseq Genome Database (refseq_genomes) vertebrates (taxid:7742) Database Organisnm Optional Exclude +

Explanation / Answer

1.a. How many Blast Hits do you get for the sequence?  

Answer = 191

1.b. What is the sequence most similar to? Provide the top-listed sequence Accession as your answer.

Answer = AB190358.1

1.c. What is the likely SOURCE ORGANISM for this sequence?

Answer = Gallus gallus

1.d. What is the E-value for this sequence and is it significant and why?

Answer = 0.0 Yes,it is significant.

1.e. What is the Max score for this sequence?

Answer = 3535

1.f. What is the Total score for the top BLASTN search result?

Answer = 3718

1.g. What is the Query coverage for the top BLASTN search result?

Answer = 100%

1.h. What is the Ident (i.e., Identity) for the top BLASTN search result?

Answer = 100%

1.i. Are there any other results having the same E-value as this sequence? If so, how many?

Answer = 99

1.j. For the other results having the same E-value as this sequence, how many also have the same Query coverage?

Answer = 6

1.k. For those results having the same E-value and same Query coverage, do they also have the same Total score?

Answer = No