You inoculate three tubes of Simmon\'s Citrate Agar with the following species:

Question

You inoculate three tubes of Simmon's Citrate Agar with the following species: 1. Klebsiella pneumoniae 2. Escherichia coli 3. Proteus mirabilis What are your expected results after 24h incubation at 37°C?

Determine the generation time ofAeromonas hydrophilausing the OD600 data below, using the 1h and the 3h time points.

You inoculate three tubes of Simmon's Citrate Agar with the following species 1. Klebsiella pneumoniae 2. Escherichia coli 3. Proteus mirabilis What are your expected results after 24h incubation at 37°C? 1. Blue color 2. Green color 3. Blue color 1. Green color 2. Blue color 3. Green color 1. Blue color 2. Blue color 3. Green color 1. Green color 2. Green color 3. Blue color QUESTION 4 Determine the generation time ofAeromonas hydrophilausing the OD600 data below, using the 1h and the 3h time points OD600 0.45 0.62 1.05 Sampling time after beginning the culture 2h 3h O 1.-1.6h 2.-3h O 3-25m 4

Explanation / Answer

1-2

There is change in colour to blue due to change in pH which occurs due to production of CO2.

4-3

It takes approx 0.33 hours for its growth.

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