2. 10pts. You are isolating a bacterial enzyme that breaks down a specific prote
ID: 218677 • Letter: 2
Question
2. 10pts. You are isolating a bacterial enzyme that breaks down a specific protein in mice. You harvest the infected cells from mice and try to grow them in the lab in regular media at room temperature, but now the enzyme is not working. a) Using what you know about enzymes, what might have changed (give at least two thing) and how would you try to fix it? You have another guess that there might be a protein in the media that's acting as an inhibitor, but you know there's no protein that mimics the normal substrate. b) Could there be another type of inhibitor? Explain!Explanation / Answer
a) every enzyme has it optimum temperature that it works at usually its 37 deg C for mammals. And as room temperature and body temperature is not the same, the enzyme may not be working properly. Additionally cells are must be supplied with carbon dioxide while culturing cell. Cells are normally cultured in incubators supplied with 5% CO2 and set at 37 deg celsius, in a sterile environment.
b) Yes the enzyme may be inhibited allosterically by molecules which do not need to mimic the substrate and may bind at any other site on the enzyme to change the structure of the binding site so that the enzyme doesnt successfully interact with the substrate
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