Molecular Biology Problem Set 1 Name 6 PTS) In a paper from 1972 Plageman descri

Question

Molecular Biology Problem Set 1 Name 6 PTS) In a paper from 1972 Plageman described an experiment where he fed yeast a Pd. The cells took up these nucleosides and added them to their pool of nucleo be looked at which nucleotides within the RNA that was produced in theces ound the following results. (he -means that no appreciable levels of radioactivity were of RNA nucleotides. in these cells was radioactive nucleotide) found in that Radioactive nucleoside Percentage of total dty found in the RMA of the yeast ??? 53% 95% AMP GMP UMP 47% 5% Uridine tidine Guanosine Inosine Adenosine 93% 51% 65% 35% Given what we talked about in class, explain the results above. place. If there were separate nuclear and cytoplasmic pools of the RNA nucleotides which pools would be used for which roles? RNA nucleotides are usecd 6) (3 PTS) What are the roles of the RNA nucleotides, and where within the cell are those roles taking

Explanation / Answer

Ans 5. In DNA A+G/T+C= 1or ratio of pyrimidine and purine bases should have a 1:1 ratio and, more specifically, that the amount of guanine is equal to cytosine and the amount of adenine is equal to thymine.

But RNA is single strandad so ratio of pyrimidine and purine bases should not have a 1:1 ratio.

According to table in question,

CMP=53%, 95%

AMP= 7%, 51%,65%

GMP=93%, 35%, 49%

UMP=47%. 5%

Here, ratio of pyrimidine and purine bases is not 1:1. so now we say radioactive nucleoside are used in RNA formation.

Ans. 6. Ribonucleic acid (RNA) is the major biological macromolecules that are essential for all known forms of life (along with DNA and proteins). A central tendency of molecular biology that the flow of genetic information in a cell is from DNA through RNA to proteins: “DNA makes RNA makes protein”.

There 3 types of RNA present in cell. Messenger RNA (mRNA) carries the genetic information copied from DNA in the form of a series of three-base code “codon,” each of which code a particular amino acid. Transfer RNA (tRNA) is the key to deciphering the code words in mRNA. Ribosomal RNA (rRNA) associates with a set of proteins to form ribosomes.

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