You perform a southern blot on ClaI-digested chromosomal DNA extracted from five

Question

You perform a southern blot on ClaI-digested chromosomal DNA extracted from five different streptomycin resistant colonies to identify which has undergone a transformation event at the metA locus. Which colony(ies) has(have) the desired genotype?

Select one:

A. Colony 1

B. Colony 2

C. Colony 3

D. Colony 4

E. Colony 5

F. Colony 4 and colony 5

G. None of these colonies

G. oilerensis oilerensis meth-km G. Colony 1 Colony 2 Colony 3 Colony 4 Colony 5 oilerensis G. G. oilerensis metA-km Colony 1 Colony 2 Colony 3 Colony 4 Colony 5

Explanation / Answer

ANS. colony 4. Here G.oilerensis is a norma strain and G.oilerensis metA-km is a transformed strain both are taken as test colonys .in the above banding image we can see same banding at colony4 with respective to transformed G.olierensis metA-km.

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