Below is a diagram of DNA replication as currently believed to occur in E.coli f

Question

Below is a diagram of DNA replication as currently believed to occur in E.coli from numbers and end at specific points. Answer the questions relating to the location specified by the numbers in the picture. (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA? (4) Which nucleic acid is probably depicted here? (5) What are these short DNA fragments usually called? (6) Which enzyme probably functions here to couple these two newly DNA? (7) Is this strand the leading or lagging strand? (5' or 3') of the molecule is here?

Explanation / Answer

1 5' end as the two strands of DNA are arranged antiparallel to one another.

2) Topoisomerases is the enzyme responsible for supercoiling of DNA.

3)DNA helicase

DNA helicase unwinds the double helix of DNA molecule into a Y-shaped fork known as the replication fork.

4) phosphate

The 5'end of DNA bears the phosphate group.

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