Below is a diagram of a DNA replication fork in E. coli . Answer the following q

Question

Below is a diagram of a DNA replication fork in E. coli. Answer the following questions relating to the parts of the model indicated by the numbered arrows.

_________5’______ 1. Is this the 5' or the 3' end of the chain?

________3’______ 2. Is this the 5' or the 3' end of the chain?

______________ 3. What is the role of this enzyme found at the replication fork?

_______________ 4. What is the name of the proteins that keep the template strands separated?

         _______________ 5. What is the name of the enzyme that releases the supercoiling caused by strand separation?

          _______________ 6a. What kind of molecule is used as a primer for new DNA strands?

          _______________ 6b. What is the name of the enzyme that makes the primer for DNA synthesis?

_______________ 7. Is the synthesis of new DNA on this strand continuous or

discontinuous?

_______________ 8. What are the short fragments of newly synthesized DNA called?

_______________ 9. What is the name of the enzyme that seals the nicks between these fragments?

Explanation / Answer

1. Is this the 5' or the 3' end of the chain?

This is the 5’ end

2. Is this the 5' or the 3' end of the chain?

This is the 3’ end

3. What is the role of this enzyme found at the replication fork?

DNA helicase is involved in DNA strand separation

4. What is the name of the proteins that keep the template strands separated?

Single-stranded binding proteins -help keep strands of DNA that have been separated

5. What is the name of the enzyme that releases the supercoiling caused by strand separation?

Topoisomerase, participate in the overwinding or underwinding of DNA

6a. What kind of molecule is used as a primer for new DNA strands?

RNA is used as a primer for new DNA strands

6b. What is the name of the enzyme that makes the primer for DNA synthesis?

RNA primase

7. Is the synthesis of new DNA on this strand continuous or discontinuous?

Leading strand is synthesized in Continuous manner

8. What are the short fragments of newly synthesized DNA called?

Okazaki fragments

9. What is the name of the enzyme that seals the nicks between these fragments?

DNA ligase seals the nicks between these fragments

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