Assuming 1. Amino acid 212 is changed from Glycine to Alanine in warm adapted sp

Question

Assuming

1.

Amino acid 212 is changed from Glycine to Alanine in warm adapted species.

Amino acid 216 is changed from Glutamine to Lysine in warm adapted species.

Amino acid 219 is changed from Theornine to Alanine in warm adapted species.

Amino acid 220 is changed from Asparatie to Glutamate warm adapted species.

2.

Both Glycine and Alanine are non polar amino acids.

Glutamine is uncharged while lysine is positively charged

Threonine is polar uncharged while Alanine is hydrophobic

Both Asparatie and Glutamate are negatively charged.

3.

The change would likely have no effect as both are non polar and hydrophobic.

There would be change as a uncharged amino acid is being replaced with positively charged.

There would be change as a polar charged amino acid is being replaced with a hydrophobic amino acid.

There would be no effect with this change as both are negatively charged amino acids.

My Question is that: Assuming that the mutations are adaptive, how should the changes that you identified affect the stability of the protein at low and high temperatures?

Explanation / Answer

Mutation are adaptive means these mutation are positive and does not decrease the stability of the protein. But when we want to see the effect of these mutation at high and low temperature, the satbiliyof the proteins decreases. This is because, as all proteins require optimal temperature for it's activity. When we increased the temperature.,bonds in protein will start to break and thus stability decreases. Similarly at low temperature, protein activity decreases.

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