Need these three details for the second part of the question\"Describe the expec

Question

Need these three details for the second part of the question"Describe the expected growth on each six plate below". Everything else is answered.

1. Type of growth (Lawn Vs Colonies)

2. Color of growth under U.V light.

3. Transformed / Untransformed cell.

Calcium chloride. It is used as salt in transformation procedure, it increases the ability of prokaryotic cell to incorporate plasmid DNA.

b) 1) LB+ plate - both tranformed and untransformed grow, because there are no antibiotics, and presenece of nutrient growth medium.

2) The tranformed E.coli would grow. Transformed cell has ampicilin resistance gene, and it comes from plasmid.

3) Both tranformed and untransformed grow. The resistance for nalidixic acid comes from the chromosomal DNA.

4) LB - no growth. Because there is no nutrient growth medium for the growth of bacteria.

5) LB amp- growth of both tranformed and untransformed. Without ampicilin (amp-) both cells will grow.

6) Growth of both tranformed and untransformed. Without nalidixic acid, both cells will grow.

You just completed a transformation plasmid. LMN2 is a strain of E. coli that has been made competent by the inclusion of a salt in an artificial competency protocol Name the salt used: LMN2 is naturally resistant to Nalidixic Acid due to chromosomal Naladixic AcidR. Cell: Plasmid: pTer contains the following OR? pTer GF Ampicillin Describe the expected growth on each of the six plates below. Describe what you would see and if there is growth, indicate why the growth was able to occur on the plate (specify where the resistance is coming from-chromosomal or plasmid) LB + LB Amp+ LB Naladixic Acid + LB- LB Amp- LB Naladixic Acid -

Explanation / Answer

1) Name of the salt used - Calcium Chloride.

2) a) we will get lawn because both transformed and the untransformed cell will grow.

b) only transformant will grow. we will get colonies.

c) we will get lawn because both transformed and the untransformed cell will grow. and will glow under UV light.

d) no growth at all because no nutrient is available.

e) we will get lawn because both transformed and the untransformed cell will grow. and will glow under UV light.

f) we will get lawn because both transformed and the untransformed cell will grow. and will not glow under UV light.

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