You are a scicntist studying several families that have members who appear to be
ID: 255776 • Letter: Y
Question
You are a scicntist studying several families that have members who appear to be resistant to infection by Asian Bird Flu. Using these markers you are able to narrow the genetic region containing the flu resistance gene down to a region containing only a few genes. You have also established collaboration with a biochemist who, using biochemical analysis of blood samples from these families, has isolateda peptide 133 amino acids long that is more abundant in the blood of resistant family members than in the blood of those susceptible to infection. By designing PCR primers based on the inferred sequences containing the flu resistance gene, you amplify two products from human genomic DNA. One product is 400 bp long, and the other is 1300 bp long. 1) a) Given that neither of these products contains introns, which product most likely encodes this gene? (1pt) b) Why (explain briefly or show your calculation)? (1lpt)Explanation / Answer
Ans. a).The peptide responsible for resistane against flu is approx 133 amino acids long, which shows that the gene product of approx 400 bp encodes this gene.
b). Three base pairs form one codon and one codon codes for 1 amino acid. So, 133 amino acids will code by 133 codons, means by 399 bp long sequence (133X3=399) and 3 bp (one codon) will act as stop codon. So, the required gene should be approx 400 bp long.
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