8. Which of the following is NOT true of blue-white screening? A Blue colonies h

Question

8. Which of the following is NOT true of blue-white screening? A Blue colonies have a functional beta-galactosidase enzyme B. Blue colonies have the lacZ gene uninterruptec C. Blue colonies have no foreign DNA inserted into the polylinker site D. White colonies contain plasmids with an insert E. All of the above are true 9. Which of the following is the most commonly used mechanism for molecular cloning? A. Binary fission B. Conjugation C. Transduction D. Transfection E. Transformation 10. In which of the following processes bacteria take up free DNA from their surroundings? A. Binary fission B. Conjugation C. Transduction D. Transfection E. Transformation 11. What does "naturally competent" mean? A. Able to donate DNA B. Able to produce blue colonies C. Able to take up foreign DNA D. Resistant to antibiotics E. All of the above 12. F plasmids, or fertility plasmids, are transferred between bacterial cells through the process of A. Binary fission B. Conjugation C. Transduction D. Transfection E. Transformation can be used to introduce recombinant DNA into host bacterial cells 13. through a manipulation of the transduction process A. Archaea B. Bacteria C. Bacteriophages D. Fungi E. Viruses

Explanation / Answer

8-option c is correct because blue colony obtain when foreign DNA insert in host cell.

9-option e is correct because in it genetic material from surroundings insert into cell.

10-option e is correct because transformation is the uptake of genetic material from surroundings.

11-option e is correct because it is the specified ability of bacteria in natural condition or laboratory.

12-option b us correct because F plasmid is transferred by conjugation to those cell in which F plasmid is absent.

13-option e is correct because from virus genetic material is transferred to bacteria through transduction process.

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