You discovered a mouse gene with an unknown function. You do not know the locati

Question

You discovered a mouse gene with an unknown function. You do not know the location or sequence of this gene in the mice genome, but a similar gene has been isolated and sequenced in yeast. How might you determine whether this gene is essential for development in mice? Create a targeting vector using a cloned copy of the gene to develop a knockout mouse. If the gene is essential for development, no offspring homozygous for knockout will be born. Transform a yeast strain deficient for this gene with DNA fragments from a mouse genomic library to determine if a mouse clone can complement the yeast defect Engineer a transgenic mouse that overexpresses this gene. If the gene is essential for development, no offspring will be born from the transgenic mice. Create a targeting vector using a cloned copy of the gene to develop a knockout mouse. If the gene is essential for development, no offspring heterozygous for the knockout will be born Insert a cloned copy of this gene into the yeast genome. If yeast have the same phenotype as mice, the gene must be essential for development in mice

Explanation / Answer

We can determine whther this gene is essential for development in mice by:
Create a targeting vector using a cloned copy of the gene to develop a knockout mouse. If the gene is essential for development, no offspring homozygous for knockout will be born.

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