3. Which of the following is NOT a consideration when the goal is expressing euk

Question

3. Which of the following is NOT a consideration when the goal is expressing eukaryotic genes in bacteria a. Which promoter to use b. Removal of introns c. The use of the AUG start codon d. Codon usage e. Strength of ribosome binding site. 14. The advantages of translation expression system include all of the following EXCEPT: a. b. c. d. e. Multiple terminators to control transcription Strong promoters that can be regulated. Built in methods for intron removal. An optimal ribosome-binding site at an appropriate distance from the AUG All of the above ARE provided. 15. Codon usage effects arise because a. The genetic code is redundant b. Some organisms will prefer one codon whereas other organisms will prefer a different codon. c. Eukaryotic and prokaryotic organisms use completely different codons d. Cells have very little tRNA for the rare codons e. Some of the above but not all of the above are true 16. Expression vectors can help prevent toxic effects of foreign proteins. Which of the following is the best description of how one of these systems work a pBAD vectors are controlled by adding IPTG to turn on expression of the lacZ promoter b. pET vectors are controlled by the level of lactose that is added to the growth medium. c. pBAD vectors are controlled by how much arabinose is added to the growth medium. d. pET vectors are controlled by how much erythromycin is added to the growth medium. 17. The stability of recombinant proteins inside cells can be improved by a. Co-expression of molecular chaperones b. Alteration of the N-terminal amino acid c. Removal of PEST sequences d. All of the above. e. Some of the above. 18. Problems in producing secretory proteins include all of the following EXCEPT a. The protein may be secreted into the periplasmic space rather than outside the cell. b. It is far more difficult to purify proteins from the growth medium than from inside the cell c. Genes can be expressed in Bacillus which does secrete into the culture medium, but the genetics is not as well-developed d. All of the above are problems secreting proteins e. Some of the above are problems secreting proteins 19. All of the following are examples of posttranslational modifications EXCEPT a. Glycosylation such as found on cell surface proteins. b. Addition of groups such as acetyl, phosphate, sulfate, and fatty acid chains. c. d. e. The addition of leader sequences to escort proteins outside of the cell. Chemical modification to form novel amino acids. Formation of disulfide bonds. 20. Yeast are excellent choices for expression of eukaryotic proteins for all of the following reasons EXCEPT a. Like E. coli, yeast are easy and quick to grow b. Yeast are used in food production, thus are considered safe

Explanation / Answer

13) Here the correct answer is (b). Removal of introns is not a consideration eukarotic gene in bacteria because in bacterias transcription is coupled to translation so no post transcriptional intron preocessing will take place.

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