The equation we use in the lab to calculate dilutions is: C1 x V1 = C2 x V2 Wher

Question

The equation we use in the lab to calculate dilutions is: C1 x V1 = C2 x V2 Where C is concentration and V is volume. To make a solution with a concentration of C2 and a volume of V2 from a stock with a concentration C1 you just need to put these three numbers into the above equation and solve for V1, the volume of stock you will need to add. Remember to keep track of the units! If you wish to make 50 mls of a 1:10 dilution of a sample in a buffer then you would add:

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mls of the sample to ? mls of the buffer

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mls of the sample to ? mls of the buffer

Explanation / Answer

Given that we need to make a 50 mL solution of 1:10 dilution.

Therefore lets assume the stock solution concentration = C1 = 10x

hence C2 = 1x and V2 = 50 mL

Putting the values in the equation

C1 * V1 = C2 * V2,

we get

10x * V1 = 1x * 50

V1 = (1x * 50) / 10x

= 50 / 10

= 5 mL

Therefore, 5 mL of 10x stock sample has to be added to 45 mL of buffer to get a 1 : 10 dilution.

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