Q2. In a haploid organism, the DNA encoding one of the tRNAs for the amino acid

Question

Q2. In a haploid organism, the DNA encoding one of the tRNAs for the amino acid tyrosine is

mutated such that the sequence of the anticodon is now 5?-CTA-3? instead of 5?-GTA-3?. Protein

synthesis takes place but the proteins produced are abnormal. Some of the proteins produced

contained extra amino acids, while others contained fewer amino acids than normal.

Explain why some proteins contained extra amino acids [1] while others contained fewer amino

acids than normal. [1]

Part IlI Q1. The piece of RNA below includes the region that codes for the binding site for the initiator tRNA needed in translation. 0 The Genetic Code 5'-GUUUCCCGUAUACAUGCGUGCCGGGGGC-3 UUU Phe (F) UCU Ser (S) UAU Tyr (Y) UGU Cys (C) UUC UUA Leu(L) UCA UUG CUU Leu (L) | CCU Pro | CAU His(H) | CGU Arg (R) CUC UCC “ UAC UAA Stop UGA Stop UAG Stop UGG Trp (W) UGC Which amino acid will be on the tRNA that is U the first to bind to the A site of the ribosome? (a)methionine (b)arginine (c)cysteine UCG CGC" CAA Gln (Q) CGA CCG CAG CGG AGC AGG GGC CUG AUU lle ACU Thr (T) AAU Asn (N) AGU Ser (S) ACC AAC AUA AUG Met (M) | ACG . GUU Val (V) GCU Ala (A) GAU Asp (D) GGU Gly (G) GUC AAA Lys (K) AGA Arg (R) AAG valine GCC GCA GCG GAC G GUA GAA Glu (E) GGA GUG GAG

Explanation / Answer

Answer 1. According to given gene sequence Balinese will b on the tRNA that is first bind to the A site of the ribosome.

Answer. 2. Having anticodon on tRNA 5' CTA 3' instead of 5'GTA 3' protein Synthesis will be stopped as CTA antocodon is complementary to UAG that is a stop codon so truncated protein is synthesised which have fewer amount of amino acid. That why normal contains extra amino acids

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