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Biochemistry Questions: I got these questions wrong, please provide the correct

ID: 261013 • Letter: B

Question

Biochemistry Questions: I got these questions wrong, please provide the correct answers with explanations/evidence.

1.) Which of the following statements is not true of mRNA?

a.) Prokaryotic mRNAs are mainly polycistronic.

b.) Eukaryotic mRNAs are mainly monocistronic.

c.) Eukaryotic mRNAs are extensively modified after synthesis.

d.) Prokaryotic mRNAs are immediately transmitted into protein without covalent modifications. <- THIS ANSWER IS WRONG.

e.) Prokaryotic mRNAs are capped with 7-methylguanosine.

2.) Nucleosomes are composed of all of the following except _______.

a.) H2A.

b.) H2B.

c.) H3. <- THIS ANSWER IS WRONG.

d.) H4.

E.) H5.

3.) Eukaryotic initiation begins with the assembly of _________.

a.) Preinitiation complex.

b.) Cap-binding complex.

c.) Poly-binding complex. <- THIS ANSWER IS WRONG.

d.) 48S Initiation complex.

e.) Translational initiation complex.

4.) Determine the mRNA sequence for the following DNA sequence. 5'-GCCATTTCCCGTTA-3'.

a.) 5'-CGGTAAAGGGCAAT-3'. <- THIS ANSWER IS WRONG.

b.) 5'-CGGUAAAGGGCAAU-3'.

c.) 5'-TAACGGCAAATGGC-3'.

d.) 5'-UAACGGCAAAUGGC-3'.

e.) 5'-UAACGGGAAAUGGC-3'.

Explanation / Answer

1) The correct answer is option E that is prokaryotic mRNAs are capped with 7-merhyl guanosine because eukaryotic mRNAs is modified with the 5' cap and 3' poly A tail but prokaryotic mRNAs does not have these changes.

2) The correct answer is option E that is H5 as there are no histone protein called H5, all other are part of histone octamer.

3) The correct answer is option A that is preinitiation complex.

4) 5'-UAACGGGAAAUGGC-3' that is option E is the correct answer because the direction is 5' to 3' of new mRNA synthesis and that is why E is the correct answer.