Biochem: The following DNA template strand will be used for questions 1-3: 3\'-

Question

Biochem:

The following DNA template strand will be used for questions 1-3: 3'- ATATCATCAGCA-5'

1. Reverse transcriptase is able to make DNA from RNA. Given the DNA segment from above, what would be the corresponding RNA sequence?

2. Suppose a problem with synthesis of nucleotides occurs that causes deoxygination of the 3' oxygen of deoxyribose of all cytosine bases, and only cytosine bases. What would be the sequences of the replicated strands of DNA?

3. Provide a method for how the scenario in question 2 could be used to create a method of sequencing. What molecular technique is this similar to? What would the gel look like for the template strand?

4. Suppose that a deamination of a pyrimidine that is complementary to Guanine occurs in a strand of DNA. Explain the resulting problem and the process that is used to fix it.

5. Explain the properties of histones that allows for DNA to be packed so closely together. BE sure to include the role that amino acids play in this.

6. Suppose the sequence GGAUC is repeated infinitely. How many amino acids would you find? List the amino acids you would find in this motif.

7. Which of the following was improperly matched with a possible anticodon?

a. M-AUG

b. Y-AUA

c. W-ACC

d. N-UUC

e. Q-GAG

f. A and C

g. A,D, and E

8. What is a lariat intron intermediate and explain the process used to temporarily create it?

Explanation / Answer

Since this question has multiple subparts, I would answer first four parts only.

1. The RNA sequence would be UAUAGUAGUCGU

2. The sequence of the replicated strands would be 5'- TATAGTAGTC-3'. The sequence would terminate at C since there would be no more addition after that.

3. This scenario is very similar to the chain-termination or sanger method of sequencing. Here chain terminating dideoxynucleotides terminates the DNA sequence. The different terminated sequences are then run on a gel in a particluar order which reveals the sequence of the DNA.

4. This is an eaxmple of mutagenesis which is one of the examples of DNA damage. Thus, this damage would evoke DNA damage repair system.

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