What is ELSD and how does it differ from UV? Explain the 2 nd limitation and con

Question

What is ELSD and how does it differ from UV?

Explain the 2nd limitation and contrast it with Beers law? Does this make it easier or harder to quantitate the data?

What about the cycloartanol glycosides makes ELSD a better detector than UV?

What about the flavonoids makes UV a better detector than ELSD?

Why do you think that structure 8 can be used on both UV and ELSD?

There is an article titled "Quantitative determination of flavonoids and cycloartanol glycosides from aerial parts of Sutherlandia frutescens (L.) R. BR. by using LC-UV/ELSD methods and confirmation by using LC–MS method" that will help answer some of the questions, i believe.

Explanation / Answer

ELSD stands for Evaporative Light Scattering Detector. It makes a measurement of photons which are scattered from volatile and semi volatile partiles that have been dried of mobile phase through evaporation. It is independent of the light absorbing properties of molecules. While, UV light makes the detection of compounds possible at a single wavelength of 210 nm and takes into account the light absorbing properties of molecules. ELSD is used in places where compounds do not efficiently absorbUV light such as sugars etc.

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