Biochemistry: A scientist performs an experiment that uses a chemical inhibitor
ID: 262368 • Letter: B
Question
Biochemistry: A scientist performs an experiment that uses a chemical inhibitor to inhibit a kinase enzyme in a signaling pathway and this results in a decrease in signaling. If the scientist wanted to be sure that this kinase was indeed responsible for the decrease in signaling, which of the following would be the best to use?
A. Use of luciferase
B. Use of antibodies
C. Use of another inhibitor that targets a unrelated kinase.
D. Use of siRNA to the kinase mRNA in question.
E. None of the above
Explanation / Answer
To be sure that this kinase was indeed responsible for the decrease in signalling, the scientist must use antibodies.
These antibodies must be targeted for the end product of this signalling pathway.
If the chemical inhibitor, inhibits the kinase enzyme, it won't be able to bring about it's function, and form the final product. So, antibodies would not give a response.
If the chemical inhibitor, doesn't inhibit the kinase enzyme, the enzyme would be able to bring about it's function and form the final product, to which antibodies used would bind, and give response.
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