The presence of a DNA template (e.g., a product from PCR), general transcription

Question

The presence of a DNA template (e.g., a product from PCR), general transcription factors, and RNA polymerase II allows for the initiation of transcription in vitro. Explain why the initiation of transcription is not possible with only general transcription factors and RNA polymerase II using the genomic DNA as a template.

a)Genomic DNA is wrapped in nucleosomes, while template DNA from PCR is not. For remodeling or removing nucleosomes, additional proteins are required.

b) Template DNA from PCR is wrapped in nucleosomes, while genomic DNA is not. The nucleosomes are required for initiation of transcription.

c) None of the above.

b) Template DNA from PCR is wrapped in nucleosomes, while genomic DNA is not. The nucleosomes are required for initiation of transcription.

c) None of the above.

Explanation / Answer

The presence of a DNA template (e.g., a product from PCR), general transcription

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