1) You have integrated the wildtype yeast URA1 gene into random chromosomal loca

Question

1) You have integrated the wildtype yeast URA1 gene into random chromosomal locations of Mat alpha budding yeast cells that cannot grow on media lacking Uracil. Below are the growth characteristics of three different URA1 integrants (strains # 1-3). Strain growth on media lackingStrain growth on Potential uracil media with 5FOAchromosomal integration site slow medium fast fast medium Very slow growth 2 In the table above add the potential chromosomal integration sites of URA1. Explain your reasoning

Explanation / Answer

URA gene encodes ODCase (orotidine 5'-phosphate decarboxylase) which is an enzyme that catalyse one reaction for the synthesis of pyrimidine nuucleotides (in RNA). loss of ODCase activity leads to decrease of cell growth in uracil/uradine lack medium.

but if we add 5FOA (5-fluoroorotic acid) in the medium then ODCase convert it into a toxic compound, 5-fluorouracil (suicide inhibitor) which cause cell death.

so if the potential integration site of URA will be more then more ODCase will be produced. it means more ODCase will be converted into toxic compound in presence of 5FOA & cell growth will be very slow.

but if the potential integration site of URA will be less then less ODCase will be produced. it means less ODCase will be converted into toxic compound in presence of 5FOA & cell growth will be fast.

sr. no. strain growth on media lacking uracil strain growth on media with 5FOA potential chromosomal integration site of URA1 1 slow fast less 2 medium medium moderate 3 fast very slow growth many

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