1) You have integrated the wildtype yeast URA1 gene into random chromosomal loca
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Question
1) You have integrated the wildtype yeast URA1 gene into random chromosomal locations of Mat alpha budding yeast cells that cannot grow on media lacking Uracil. Below are the growth characteristics of three different URA1 integrants (strains # 1-3). Strain growth on media lackingStrain growth on Potential uracil media with 5FOAchromosomal integration site slow medium fast fast medium Very slow growth 2 In the table above add the potential chromosomal integration sites of URA1. Explain your reasoningExplanation / Answer
Please find the answers below:
Answer 1: Just below the promoter: The genetic sequence which are localized just after the promoter regions are highly expressed in the cells. This is because proximity of the promoter sequence enhances the binding of RNA polymerase on the cistron and thus promotes up-regulation of gene expression.
Answer 2: Distant from the promoter: As the distance of the insert increases from the promoter sequence, the binding strenght of the RNA polymerase decreases and thus, the speed and extent of gene expression decreases.
Answer 3: Near the stop-sequence or in the intronic region: Very slow growth of the gene indicates its highly distant localization with respect to the promoter, thus deciphering very slow or no growth.
Thus, the chromosomal localization of the gene determines its extent, frequency and speed of expression.
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