2. Back in lab, you treat liver cells with a high concentration of a membrane pe
ID: 267584 • Letter: 2
Question
2. Back in lab, you treat liver cells with a high concentration of a membrane permeable cAMP analog (stable molecule, similar to cAMP, that can mimic cAMP in the cel) A. (3pts) You are able to measure two downstream responses to the cAMP analog. Please circle whether these are relatively FAST or SLOW responses. Phosphorylation of PKA FAST or SLOW Transcript levels of a target gene FAST or SLOW B. (3pts) Explain your above choices and why these make most sense based on your understanding of the timescales of cellular signaling.Explanation / Answer
A. (1) Phosphorylation of PKA = FAST
(2) Transcript level of target gene = FAST
B. cAMP in the cell is formed by an enzyme adenylate cyclase, and it phosphorylate protein kinase A (PKA) in the cell and then transcription of target genes occur. So if cells treated with a compound that mimic cAMP, then the phosphorylation of PKA and transcription of target genes occure quickly.
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