GENETIC MAPPING 3. How many of the 4 haploid spores will be MAT a? How many will

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GENETIC MAPPING

3. How many of the 4 haploid spores will be MAT a? How many will be MAT a?

4. Write the genotypes of the two parent strains and the resulting diploid. Use the allele names ADE, ade, LYS, and lys. (UPPERCASE for dominant; lowercase for recessive alleles.)

5. You induce meiosis in the diploid yeast, and you collect 40 haploid spores. If these two genes assort independently, how many of the haploid spores will you expect to be able to grow on a plate without adenine and lysine (a–ade–lys plate)?

6. Out of the 40 haploid spores you collected, 5 haploid spores can grow on a –ade –lys plate and 35 haploid spores cannot grow on a –ade –lys plate. Of these 35 haploids, 15 can grow on a –ade plate, 15 can grow on a –lys plate, and 5 cannot grow on either –lys or –ade plates. Calculate the percentage of recombinant gametes you collected.

Note: The percentage of recombinant gametes detected is the map distance between the genes

ADE and LYS. As we discussed in lecture, the unit for map distance is centiMorgans(cM) or map units(m.u.).

You have decided to start an environmentally friendly beer brewery, and you learn that yeast strains used to make ale beers effectively take up mal tose, while maltose is not transported into cells ofyeast strains used to make lager beers. Because the yeast used for ales can transport maltose, ales can be produced more economically and in a more en vironmentally favorable way You would like to make lager beers in a more environmentally favorable way, so you decide to learn more about the gene responsible for maltose transport in yeast. You name the gene responsible for maltose transport AGTI (for ?-glucose transporter 1 ) A short review about yeast Yeast cells can grow and divide mitotically both when they are in a haploid state and when they are in a diploid state. So you can grow a culture of either haploid or diploid yeast. Haploid yeast cells have one of two mating types, MAT a or MAT ? (similar to having two sexes), a and ? are two different alleles of the MAT locus When a MAT a haploid and MAT ? haploid mate. they form a diploid cell. a haploid o haploid mating Under certain conditions, the diploid can undergo meiosis and produce 4 haploid spores. The spores can be separated and grown in separate tubes. (Remember that unlike human haploid cells [sperm cells and eggs] haploid yeast cells can grow mitotically.) diploid eiosis spores will be MAT a? 3. How many of the 4 haploid How many will be MAT a? O 4 haploid OO spores You mate a haploid yeast that cannot synthesize adenine with another haploid yeast that cannot synthesize lysine. Assume that each of these strains has a single recessive mutation in the loci ADE and LYS respectively. The resulting diploid can grow on a plate without adenine and lysine . Write the genotypes of the two parent strains and the resulting diploid. Use the allele names ADE, ade, LYS, and lys. (UPPERCASE for dominant; lowercase for recessive alleles.)

Explanation / Answer

3. From the 4 haploid spores there will be 2 MAT a and 2 will be MAT alpha. Because during the  process of meiosis four haploid spores are produced; out of which two are a spores and two will be ? spores.

The genotypes of two parent strains are MATa and MAT?. ADE is dominant over ade and LYS is dominant over lys.

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